Journal:

Article Title: Characterization of Non-Starter Lactic Acid Bacteria from Italian Ewe Cheeses Based on Phenotypic, Genotypic, and Cell Wall Protein Analyses

doi: 10.1128/AEM.67.5.2011-2020.2001

Figure Lengend Snippet: Characteristics fo the NSLAB type strains and isolates from Italian ewe cheeses

Article Snippet: All the L. paracasei subsp. paracasei isolates, grouped in cluster 11, which also included three isolates of L. curvatus , some unidentified isolates, and the type strain L. casei subsp. casei ATCC 334 T . The different RAPD patterns which show the representative fingerprint for each of the 11 clusters are shown in Fig. . Primers P1, P4, and P7 amplified DNA and produced bands at 250 to 4,200 bp.

Techniques:

Journal:

Article Title: Characterization of Non-Starter Lactic Acid Bacteria from Italian Ewe Cheeses Based on Phenotypic, Genotypic, and Cell Wall Protein Analyses

doi: 10.1128/AEM.67.5.2011-2020.2001

Figure Lengend Snippet: SDS-PAGE patterns of cell wall proteins from Lactobacillus isolates. Lane 1, standard proteins (see Materials and Methods); lane 2, L. fermentum 65H; lane 3, L. brevis 68E; lane 4, L. curvatus 1HD; lane 5, L. casei subsp. casei M16; lane 6, L. paracasei subsp. paracasei 4H3; lane 7, L. casei subsp. pseudoplantarum 115K; lane 8, L. plantarum 144; lane 9, L. pentosus 2HG; lane 10, L. rhamnosus 15H3.

Article Snippet: All the L. paracasei subsp. paracasei isolates, grouped in cluster 11, which also included three isolates of L. curvatus , some unidentified isolates, and the type strain L. casei subsp. casei ATCC 334 T . The different RAPD patterns which show the representative fingerprint for each of the 11 clusters are shown in Fig. . Primers P1, P4, and P7 amplified DNA and produced bands at 250 to 4,200 bp.

Techniques: SDS Page

Journal: Microbial Cell Factories

Article Title: Investigating human-derived lactic acid bacteria for alcohol resistance

doi: 10.1186/s12934-024-02375-4

Figure Lengend Snippet: Growth curve of ethanol-tolerant LAB in 0, 2%, 5%, 8%, 10%, 12% (v/v) of ethanol-added MRS broth. ( A ) L. paracasei Alc1, ( B ) L. rhamnosus AA, ( C ) P. acidilactici Alc3, ( D ) L. paracasei Alc4, ( E ) P. acidilactici Alc5

Article Snippet: Fig. 3 Hemolytic activity test. ( A ) L. paracasei Alc1 ( B ) L. rhamnosus AA ( C ) P. acidilactici Alc3 ( D ) L. paracasei Alc4 ( E ) P. acidilactici Alc5 ( F ) Listeria ivanovii subsp. ivanovii ATCC 19,119 (positive control) Antibiotic resistance genes in a probiotic genome can be transferred to pathogenic bacteria to generate antibiotic-resistant pathogens [ , ].

Techniques:

Journal: Microbial Cell Factories

Article Title: Investigating human-derived lactic acid bacteria for alcohol resistance

doi: 10.1186/s12934-024-02375-4

Figure Lengend Snippet: Growth curves ( A ) and survival rates ( B ) of ethanol-tolerant LAB in Makgeolli ( A , B ) and Baekseju ( C , D ). Alc1, L. paracasei Alc1; LAA, L. rhamnosus AA; Alc3, P. acidilactici Alc3; Alc4, L. paracasei Alc4; Alc5, P. acidilactici Alc5. Treatments with different letters are significantly different at p < 0.05

Article Snippet: Fig. 3 Hemolytic activity test. ( A ) L. paracasei Alc1 ( B ) L. rhamnosus AA ( C ) P. acidilactici Alc3 ( D ) L. paracasei Alc4 ( E ) P. acidilactici Alc5 ( F ) Listeria ivanovii subsp. ivanovii ATCC 19,119 (positive control) Antibiotic resistance genes in a probiotic genome can be transferred to pathogenic bacteria to generate antibiotic-resistant pathogens [ , ].

Techniques:

Journal: Microbial Cell Factories

Article Title: Investigating human-derived lactic acid bacteria for alcohol resistance

doi: 10.1186/s12934-024-02375-4

Figure Lengend Snippet: Bacterial ammonia production

Article Snippet: Fig. 3 Hemolytic activity test. ( A ) L. paracasei Alc1 ( B ) L. rhamnosus AA ( C ) P. acidilactici Alc3 ( D ) L. paracasei Alc4 ( E ) P. acidilactici Alc5 ( F ) Listeria ivanovii subsp. ivanovii ATCC 19,119 (positive control) Antibiotic resistance genes in a probiotic genome can be transferred to pathogenic bacteria to generate antibiotic-resistant pathogens [ , ].

Techniques:

Journal: Microbial Cell Factories

Article Title: Investigating human-derived lactic acid bacteria for alcohol resistance

doi: 10.1186/s12934-024-02375-4

Figure Lengend Snippet: Hemolytic activity test. ( A ) L. paracasei Alc1 ( B ) L. rhamnosus AA ( C ) P. acidilactici Alc3 ( D ) L. paracasei Alc4 ( E ) P. acidilactici Alc5 ( F ) Listeria ivanovii subsp. ivanovii ATCC 19,119 (positive control)

Article Snippet: Fig. 3 Hemolytic activity test. ( A ) L. paracasei Alc1 ( B ) L. rhamnosus AA ( C ) P. acidilactici Alc3 ( D ) L. paracasei Alc4 ( E ) P. acidilactici Alc5 ( F ) Listeria ivanovii subsp. ivanovii ATCC 19,119 (positive control) Antibiotic resistance genes in a probiotic genome can be transferred to pathogenic bacteria to generate antibiotic-resistant pathogens [ , ].

Techniques: Activity Assay, Positive Control

Journal: Microbial Cell Factories

Article Title: Investigating human-derived lactic acid bacteria for alcohol resistance

doi: 10.1186/s12934-024-02375-4

Figure Lengend Snippet: Antimicrobial susceptibility of the LAB isolates

Article Snippet: Fig. 3 Hemolytic activity test. ( A ) L. paracasei Alc1 ( B ) L. rhamnosus AA ( C ) P. acidilactici Alc3 ( D ) L. paracasei Alc4 ( E ) P. acidilactici Alc5 ( F ) Listeria ivanovii subsp. ivanovii ATCC 19,119 (positive control) Antibiotic resistance genes in a probiotic genome can be transferred to pathogenic bacteria to generate antibiotic-resistant pathogens [ , ].

Techniques:

Journal: Microbial Cell Factories

Article Title: Investigating human-derived lactic acid bacteria for alcohol resistance

doi: 10.1186/s12934-024-02375-4

Figure Lengend Snippet: Growth curve of ethanol-tolerant LAB with different carbon sources. ( A ) L. paracasei Alc1, ( B ) L. rhamnosus AA, ( C ) P. acidilactici Alc3, ( D ) L. paracasei Alc4, ( E ) P. acidilactici Alc5. Glu, glucose; Fru, fructose; Gal, galactose; FOS, fructooligosaccharides; B-FOS, butyl-fructooligosaccharides; 2-FL, 2-fucosyllactose; Suc, sucrose; Lac, lactose

Article Snippet: Fig. 3 Hemolytic activity test. ( A ) L. paracasei Alc1 ( B ) L. rhamnosus AA ( C ) P. acidilactici Alc3 ( D ) L. paracasei Alc4 ( E ) P. acidilactici Alc5 ( F ) Listeria ivanovii subsp. ivanovii ATCC 19,119 (positive control) Antibiotic resistance genes in a probiotic genome can be transferred to pathogenic bacteria to generate antibiotic-resistant pathogens [ , ].

Techniques:

Journal: Microbial Cell Factories

Article Title: Investigating human-derived lactic acid bacteria for alcohol resistance

doi: 10.1186/s12934-024-02375-4

Figure Lengend Snippet: Adhesion ability of the selected LAB strains to Caco-2 cells monolayer with different carbon sources. ( A ) L. paracasei Alc1, ( B ) L. rhamnosus AA, ( C ) P. acidilactici Alc3, ( D ) L. paracasei Alc4, ( E ) P. acidilactici Alc5. Con, control; Glu, glucose; Fru, fructose; Gal, galactose; FOS, fructooligosaccharides; B-FOS, butyl-fructooligosaccharides; 2-FL, 2-fucosyllactose; Suc, sucrose; Lac, lactose. Treatments with different letters are significantly different at p < 0.05

Article Snippet: Fig. 3 Hemolytic activity test. ( A ) L. paracasei Alc1 ( B ) L. rhamnosus AA ( C ) P. acidilactici Alc3 ( D ) L. paracasei Alc4 ( E ) P. acidilactici Alc5 ( F ) Listeria ivanovii subsp. ivanovii ATCC 19,119 (positive control) Antibiotic resistance genes in a probiotic genome can be transferred to pathogenic bacteria to generate antibiotic-resistant pathogens [ , ].

Techniques: Control

Journal: Microbial Cell Factories

Article Title: Investigating human-derived lactic acid bacteria for alcohol resistance

doi: 10.1186/s12934-024-02375-4

Figure Lengend Snippet: IL-8 production in lipopolysaccharide (LPS)-stimulated Caco-2 cells when co-cultured with L. paracasei Alc1 ( A ), L. rhamnosus AA ( B ), P. acidilactici Alc3 ( C ), P. acidilactici Alc5 ( D ) using different carbon sources. Con, control; LPS, LPS-treated cells; Glu, glucose; Fru, fructose; Gal, galactose; FOS, fructooligosaccharides; B-FOS, butyl-fructooligosaccharides; 2-FL, 2-fucosyllactose; Suc, sucrose; Lac, lactose. Treatments with different letters are significantly different at p < 0.05

Article Snippet: Fig. 3 Hemolytic activity test. ( A ) L. paracasei Alc1 ( B ) L. rhamnosus AA ( C ) P. acidilactici Alc3 ( D ) L. paracasei Alc4 ( E ) P. acidilactici Alc5 ( F ) Listeria ivanovii subsp. ivanovii ATCC 19,119 (positive control) Antibiotic resistance genes in a probiotic genome can be transferred to pathogenic bacteria to generate antibiotic-resistant pathogens [ , ].

Techniques: Cell Culture, Control

Journal: Microbial Cell Factories

Article Title: Investigating human-derived lactic acid bacteria for alcohol resistance

doi: 10.1186/s12934-024-02375-4

Figure Lengend Snippet: IL-8 production in lipopolysaccharide (LPS)-stimulated HT-29 cells when co-cultured with L. paracasei Alc1 ( A ), L. rhamnosus AA ( B ), P. acidilactici Alc3 ( C ), P. acidilactici Alc5 ( D ) using different carbon sources. Con, control; LPS, LPS-treated cells; Glu, glucose; Fru, fructose; Gal, galactose; FOS, fructooligosaccharides; B-FOS, butyl-fructooligosaccharides; 2-FL, 2-fucosyllactose; Suc, sucrose; Lac, lactose. Treatments with different letters are significantly different at p < 0.05

Article Snippet: Fig. 3 Hemolytic activity test. ( A ) L. paracasei Alc1 ( B ) L. rhamnosus AA ( C ) P. acidilactici Alc3 ( D ) L. paracasei Alc4 ( E ) P. acidilactici Alc5 ( F ) Listeria ivanovii subsp. ivanovii ATCC 19,119 (positive control) Antibiotic resistance genes in a probiotic genome can be transferred to pathogenic bacteria to generate antibiotic-resistant pathogens [ , ].

Techniques: Cell Culture, Control

Journal: Microorganisms

Article Title: Effect of Fluidized Bed Drying, Matrix Constituents and Structure on the Viability of Probiotic Lactobacillus paracasei ATCC 55544 during Storage at 4 °C, 25 °C and 37 °C

doi: 10.3390/microorganisms10010074

Figure Lengend Snippet: The storage stability of fluidized bed-dried Lactobacillus paracasei powders embedded in whole milk powder matrix with ( a ) a w 0.3, ( d ) a w 0.4, ( g ) a w 0.5; skim milk powder matrix with ( b ) a w 0.3, ( e ) a w 0.4, ( h ) a w 0.5; milk protein isolate matrix with ( c ) a w 0.3, ( f ) a w 0.4, ( i ) a w 0.5. The viability is expressed as the logarithmic values of survival against a storage time of 4 weeks at 4 °C (black circle), 25 °C (white triangle), and 37 °C (black square). Error bars represent the standard deviation of means ( n ≥ 3).

Article Snippet: This biofilm-like structure and the protective effect may possibly be enhanced by the presence of exopolysaccharides and the adhesive properties of L. paracasei ATCC 55544 [ , ].

Techniques: Standard Deviation

Journal: Microorganisms

Article Title: Effect of Fluidized Bed Drying, Matrix Constituents and Structure on the Viability of Probiotic Lactobacillus paracasei ATCC 55544 during Storage at 4 °C, 25 °C and 37 °C

doi: 10.3390/microorganisms10010074

Figure Lengend Snippet: Scanning Electron Microscopy (SEM) images of fluidized bed-dried Lactobacillus paracasei powders in MPI ( a ), SMP ( b ) or WMP ( c ).

Article Snippet: This biofilm-like structure and the protective effect may possibly be enhanced by the presence of exopolysaccharides and the adhesive properties of L. paracasei ATCC 55544 [ , ].

Techniques: Electron Microscopy

Journal: Microorganisms

Article Title: Effect of Fluidized Bed Drying, Matrix Constituents and Structure on the Viability of Probiotic Lactobacillus paracasei ATCC 55544 during Storage at 4 °C, 25 °C and 37 °C

doi: 10.3390/microorganisms10010074

Figure Lengend Snippet: CLSM image of ( a ) surface of the WMP matrix, showing the immobilization of probiotic Lactobacillus paracasei within the fat and protein layers ( b ) cross section of the matrix, ( c ) spatial distribution of live and dead bacteria which are located below the surface, comprising of bacteria embedded in the protein and lactose matrix. Green live bacteria, red dead bacteria. The scale bar represents 20 µm.

Article Snippet: This biofilm-like structure and the protective effect may possibly be enhanced by the presence of exopolysaccharides and the adhesive properties of L. paracasei ATCC 55544 [ , ].

Techniques: Bacteria